Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

Daniel A. Harki; Jason D. Graci; Jocelyn P. Edathil; Christian Castro; Craig E. Cameron; Blake R. Peterson

doi:10.1002/cbic.200700160

Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

Daniel A. Harki, Jason D. Graci, Jocelyn P. Edathil, Christian Castro, Craig E. Cameron, Blake R. Peterson

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

(Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.

Original language	English (US)
Pages (from-to)	1359-1362
Number of pages	4
Journal	ChemBioChem
Volume	8
Issue number	12
DOIs	https://doi.org/10.1002/cbic.200700160
State	Published - Aug 13 2007

Keywords

Antiviral agents
Mutagenesis
Nucleobases
Poliovirus
Polymerases
Universal base

Access

10.1002/cbic.200700160

OpenUrl availability

Full text

Cite this

@article{bf25ab24ff8e4d4a9776e1ab064edc16,

title = "Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase",

abstract = "(Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.",

keywords = "Antiviral agents, Mutagenesis, Nucleobases, Poliovirus, Polymerases, Universal base",

author = "Harki, {Daniel A.} and Graci, {Jason D.} and Edathil, {Jocelyn P.} and Christian Castro and Cameron, {Craig E.} and Peterson, {Blake R.}",

year = "2007",

month = aug,

day = "13",

doi = "10.1002/cbic.200700160",

language = "English (US)",

volume = "8",

pages = "1359--1362",

journal = "ChemBioChem",

issn = "1439-4227",

publisher = "Wiley-VCH Verlag",

number = "12",

}

TY - JOUR

T1 - Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

AU - Harki, Daniel A.

AU - Graci, Jason D.

AU - Edathil, Jocelyn P.

AU - Castro, Christian

AU - Cameron, Craig E.

AU - Peterson, Blake R.

PY - 2007/8/13

Y1 - 2007/8/13

N2 - (Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.

AB - (Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.

KW - Antiviral agents

KW - Mutagenesis

KW - Nucleobases

KW - Poliovirus

KW - Polymerases

KW - Universal base

UR - http://www.scopus.com/inward/record.url?scp=34548124606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548124606&partnerID=8YFLogxK

U2 - 10.1002/cbic.200700160

DO - 10.1002/cbic.200700160

M3 - Article

C2 - 17600792

AN - SCOPUS:34548124606

SN - 1439-4227

VL - 8

SP - 1359

EP - 1362

JO - ChemBioChem

JF - ChemBioChem

IS - 12

ER -

Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

Abstract

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this