Synthesis of Arylazide- and Diazirine-Containing CrAsH-EDT2 Photoaffinity Probes

Shameem S. Syeda, Daren Rice, Derek J. Hook, Leslie L. Heckert, Gunda I. Georg

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Two photo-crosslinking biarsenical (CrAsH-EDT2)-modified probes were synthesized that are expected to be useful tools for tetracysteine-labeled proteins to facilitate the co-affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH-EDT2 and N1-(4-azido-2-nitrophenyl)hexane-1,6-diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3-related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalArchiv der Pharmazie
Volume349
Issue number4
DOIs
StatePublished - Apr 1 2016

Keywords

  • Biarsenical probe
  • Fluorescence
  • Photoaffinity probes
  • Tetracysteine-tagged recombinant DMRT1

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