TACAN Is an Ion Channel Involved in Sensing Mechanical Pain

Lou Beaulieu-Laroche, Marine Christin, Annmarie Donoghue, Francina Agosti, Noosha Yousefpour, Hugues Petitjean, Albena Davidova, Craig Stanton, Uzair Khan, Connor Dietz, Elise Faure, Tarheen Fatima, Amanda MacPherson, Stephanie Mouchbahani-Constance, Daniel G. Bisson, Lisbet Haglund, Jean A. Ouellet, Laura S. Stone, Jonathan Samson, Mary Jo SmithKjetil Ask, Alfredo Ribeiro-da-Silva, Rikard Blunck, Kate Poole, Emmanuel Bourinet, Reza Sharif-Naeini

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

TACAN is identified as an ion channel that contributes to mechanosensitive currents in nociceptors and detecting mechanical pain stimuli.

Original languageEnglish (US)
Pages (from-to)956-967.e17
JournalCell
Volume180
Issue number5
DOIs
StatePublished - Mar 5 2020
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by operating grants from the Canadian Institutes of Health Research, a pilot grant from the Groupe d'Etude des Prot?ines Membranaires (GEPROM), and a salary award from the Fonds de Recherche du Quebec-Sant? (to R.S.-N.). E.B. was supported by grants from the Fondation pour la Recherche M?dicale (?quipe FRM 2015) and the Agence Nationale pour la Recherche (ANR15-CE16-0012-01 and LABEX ICST). A.R.-d.-S. was supported by an operating grant from the Canadian Institutes of Health Research (MOP-136903). K.P. was partially supported by NHMRC project grant APP1122104. M.C. and N.Y. were supported by fellowships from the Louise and Alan Edwards Foundation. L.B.-L. is supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) (PGSD2-517068-2018). K.A. was supported by the Ontario Thoracic Society and the Canada Foundation for Innovation John R. Evans Leaders Fund (31979). L.B.-L. performed and analyzed electrophysiological recordings in heterologous cell lines and nociceptors. He also contributed to manuscript and figure preparation. M.C. performed and analyzed electrophysiological recordings in heterologous cell lines and behavior experiments. A. Donoghue performed bilayer experiments. F.A. and E.B. performed whole-cell DRG recordings. N.Y. T.F. and A.R.-d.-S. performed immunohistochemistry experiments. H.P. performed in situ hybridization and behavior experiments. A. Davidova performed in situ experiments, immunostaining, cell culture, and transfection; validated siRNA and shRNA; and generated stable cell lines. C.S. performed COS-7 recordings. U.K. and C.D. performed HEK293 recordings. E.F. performed TACAN purification experiments for bilayer recordings. A.M. performed TACAN membrane localization experiments. S.M.-C. D.G.B. L.H. J.A.O. and L.S.S. dissected human DRG from donors. J.S. provided in situ probes for human TACAN. M.-J.S. and K.A. performed ISH on human DRGs. R.B. supervised bilayer experiments. K.P. performed whole-cell TACAN experiments on pillars. R.S.-N. designed experiments, supervised the project, and prepared the manuscript. R.S.-N. has a patent (US9551718B2) related to this work.

Funding Information:
This work was supported by operating grants from the Canadian Institutes of Health Research , a pilot grant from the Groupe d’Etude des Protéines Membranaires (GEPROM), and a salary award from the Fonds de Recherche du Quebec-Santé (to R.S.-N.). E.B. was supported by grants from the Fondation pour la Recherche Médicale ( Équipe FRM 2015 ) and the Agence Nationale pour la Recherche ( ANR15-CE16-0012-01 and LABEX ICST ). A.R.-d.-S. was supported by an operating grant from the Canadian Institutes of Health Research ( MOP-136903 ). K.P. was partially supported by NHMRC project grant APP1122104 . M.C. and N.Y. were supported by fellowships from the Louise and Alan Edwards Foundation . L.B.-L. is supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) ( PGSD2-517068-2018 ). K.A. was supported by the Ontario Thoracic Society and the Canada Foundation for Innovation John R. Evans Leaders Fund ( 31979 ).

Publisher Copyright:
© 2020

Keywords

  • TACAN
  • bilayer
  • ion channel
  • mechanosensitive
  • mechanotransduction
  • nociceptor
  • pain
  • patch clamp
  • pillar

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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