@inbook{b48d0f5e3e9940be9c36ee9542534a45,
title = "Tailor-made mutations in arabidopsis using zinc finger nucleases",
abstract = "Zinc finger nucleases (ZFNs) are proteins engineered to make site-specific double-strand breaks (DSBs) in a DNA sequence of interest. Imprecise repair of the ZFN-induced DSBs by the nonhomologous end-joining (NHEJ) pathway results in a spectrum of mutations, such as nucleotide substitutions, insertions, and deletions. Here we describe a method for targeted mutagenesis in Arabidopsis with ZFNs, which are engineered by context-dependent assembly (CoDA). This ZFN-induced mutagenesis method is an alternative to other currently available gene knockout or knockdown technologies and is useful for reverse genetic studies.",
keywords = "Arabidopsis, CoDA, Mutagenesis, NHEJ, ZFN",
author = "Yiping Qi and Starker, {Colby G.} and Feng Zhang and Baltes, {Nicholas J.} and Voytas, {Daniel F.}",
year = "2014",
doi = "10.1007/978-1-62703-580-4_10",
language = "English (US)",
isbn = "9781627035798",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "193--209",
booktitle = "Arabidopsis Protocols",
}