Tantalus, a novel ASX-interacting protein with tissue-specific functions

Bruce H. Dietrich; Jocelyn Moore; Michael Kyba; Gilbert DosSantos; Fiona McCloskey; Thomas A. Milne; Hugh W. Brock; Henry M. Krause

doi:10.1006/dbio.2001.0255

Tantalus, a novel ASX-interacting protein with tissue-specific functions

Bruce H. Dietrich, Jocelyn Moore, Michael Kyba, Gilbert DosSantos, Fiona McCloskey, Thomas A. Milne, Hugh W. Brock, Henry M. Krause

Pediatrics - Blood/Marrow Transplant

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The Drosophila trithorax- and Polycomb-group (trxG and PcG) proteins maintain activated and repressed transcriptional states at specific target gene loci. The Additional sex combs (Asx) gene is of particular interest as it appears to function in both protein complexes and yet its effects on target genes are more restricted. A novel protein, Tantalus (TAN), was identified in a yeast two-hybrid screen for ASX-interacting proteins that might confer tissue-specific ASX functions. TAN contains consensus nuclear localization sites and binds DNA in vitro. However, its subcellular localization varies in a tissue-specific fashion. In salivary glands, TAN is predominantly nuclear and associates with 66 euchromatic sites on polytene chromosomes, more than half of which overlap with ASX. These loci do not include the homeotic genes of the ANT and BX complexes bound by other PcG and trxG proteins. Rather, tan mutant defects are restricted to sensory organs. We show that one of these defects, shared by Asx, is genetically enhanced by Asx. Taken together, the data suggest that TAN is a tissue-specific cofactor for ASX, and that its activity may be partially controlled by subcellular trafficking.

Original language	English (US)
Pages (from-to)	441-453
Number of pages	13
Journal	Developmental Biology
Volume	234
Issue number	2
DOIs	https://doi.org/10.1006/dbio.2001.0255
State	Published - Jun 15 2001

Bibliographical note

Funding Information:
We thank Carl Hashimoto, Kathy Matthews and the Umea Stock Center for fly stocks, Shelley Lumba for assistance with antibody characterization, and Howard Lipshitz for comments on the manuscript. This work was supported by funds provided by the Canadian Institutes of Health Research (to H.M.K. and H.W.B.), the Natural Sciences and Engineering Research Council (to H.W.B.) and a University of Toronto Open Fellowship (to B.H.D.). tan sequence data has been submitted to the DDBJ/EMBL/GenBank databases under accession number AF218585.

Keywords

Additional sex combs
Drosophila
Polycomb
Sensory organs
Tantalus
Tissue-specific
Trithorax

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title = "Tantalus, a novel ASX-interacting protein with tissue-specific functions",

abstract = "The Drosophila trithorax- and Polycomb-group (trxG and PcG) proteins maintain activated and repressed transcriptional states at specific target gene loci. The Additional sex combs (Asx) gene is of particular interest as it appears to function in both protein complexes and yet its effects on target genes are more restricted. A novel protein, Tantalus (TAN), was identified in a yeast two-hybrid screen for ASX-interacting proteins that might confer tissue-specific ASX functions. TAN contains consensus nuclear localization sites and binds DNA in vitro. However, its subcellular localization varies in a tissue-specific fashion. In salivary glands, TAN is predominantly nuclear and associates with 66 euchromatic sites on polytene chromosomes, more than half of which overlap with ASX. These loci do not include the homeotic genes of the ANT and BX complexes bound by other PcG and trxG proteins. Rather, tan mutant defects are restricted to sensory organs. We show that one of these defects, shared by Asx, is genetically enhanced by Asx. Taken together, the data suggest that TAN is a tissue-specific cofactor for ASX, and that its activity may be partially controlled by subcellular trafficking.",

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AU - Milne, Thomas A.

AU - Brock, Hugh W.

AU - Krause, Henry M.

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Tantalus, a novel ASX-interacting protein with tissue-specific functions

Abstract

Bibliographical note

Keywords

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