Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses

Nicolas Van Nieuwenhove, Éric Potvin, Maija Heikkilä, Vera Pospelova, Kenneth Neil Mertens, Edwige Masure, Małgorzata Kucharska, Eun Jin Yang, Nicolas Chomérat, Marek Zajaczkowski

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SEM) observations of cysts from Barents Sea surface sediments, and genetic analyses of cysts from the Beaufort Sea. The measurements suggest that populations and morphospecies cannot be distinguished based on morphometric criteria. Furthermore, sequential sediment trap samples from Hudson Bay reveal that morphological variation can occur at the same location over a few weeks, arguing against a uniform morphological response to environmental parameters. The SEM observations reveal a consistent gonyaulacacean tabulation (Po, 4′, 6′′, 6c, 5 s, *6′′′, 1p, 1′′′′). The small and large subunit ribosomal RNA genes and the internal transcribed spacer sequences obtained from differing cysts from the Beaufort Sea with morphologies attributable to the complex, including forms that correspond to Rottnestia amphicavata, were all identical and conform to those of Gonyaulax elongata from the Orkney Islands. The molecular analyses thus support the conclusion that the morphological variability is not reflected genetically and occurs within one species. Based on arguments against the generic attribution of amphicavata to Rottnestia, the continuum between the extreme ends of the morphological range, and the molecular data, we suggest Rottnestia amphicavata to be conspecific with Spiniferites frigidus, and both to be junior synonyms of Spiniferites elongatus. The morphometric data further indicate that Spiniferites ellipsoideus, an elongate cyst from the Miocene, can also be considered a junior synonym of Spiniferites elongatus. It is recommended to use two informal types in census work (i.e. Spiniferites elongatus–Beaufort morphotype for morphologies formerly assignable to Spiniferites frigidus/Rottnestia amphicavata, and Spiniferites elongatus–Norwegian morphotype for cysts with strongly reduced processes) to separate specimens at both extreme ends of the morphological spectrum from typical specimens of Spiniferites elongatus.

Original languageEnglish (US)
Pages (from-to)111-134
Number of pages24
JournalPalynology
Volume42
Issue numbersup1
DOIs
StatePublished - Dec 20 2018
Externally publishedYes

Bibliographical note

Funding Information:
Funding to NVN was provided by the Danish Council for Independent Research, Natural Science [project no. 12–126709/FNU]. The genetic work by EP was supported by the K-PORT [Korea-Polar Ocean in Rapid Transition, KOPRI, PM15040] and K-POD [Korea-Polar Ocean Development, KOPRI, PM15050] projects funded by the Ministry of Oceans and Fisheries of Korea. This research was partially funded by the Natural Sciences and Engineering Research Council of Canada (NSERC) through a Discovery grant to VP. She is the Hanse-Wissenschaftskolleg (HWK) senior research fellow in marine and climate research at the Institute for Advanced Study (Germany). Funding for MH was provided by the Academy of Finland [grant 252512] and the Villlum Foundation, Denmark [VKR 023454].

Publisher Copyright:
© 2018, © 2018 The Author(s). Published by AASP–The Palynological Society.

Keywords

  • Single-cell PCR
  • Spiniferites ellipsoideus; Spiniferites frigidus; Rottnestia amphicavata
  • morphologic variability
  • taxonomy

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