Abstract
To gain insights into how TGF-β regulates epithelial-mesenchymal transition (EMT), we assessed the time course of proteins and mRNAs during EMT by multiplex iTRAQ labeling and 2D-LC-MS/MS, and by hybridization, respectively. Temporal iTRAQ analysis identified 66 proteins as differentially expressed during EMT, including newly associated proteins calpain, fascin and macrophage-migration inhibitory factor (MIF). Comparing protein and mRNA expression overtime showed that all the 14 up-regulated proteins involved in the actin-cytoskeleton remodeling were accompanied by increases in corresponding mRNA expression. Interestingly, siRNA mediated knockdown of cofilinl potentiated TGFβ-induced EMT. Further analysis of cofilinl and β-actin revealed an increase in their mRNA stability in response to TGF-β, contributing to the observed increase in mRNA and protein expression. These results are the first demonstration of post-transcriptional regulation of cytoskeletal remodelling and a key role for cofilinl during TGF-β-induced EMT.
Original language | English (US) |
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Pages (from-to) | 35-47 |
Number of pages | 13 |
Journal | Journal of Proteome Research |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2009 |
Keywords
- Actin-cytoskeleton remodeling
- Beta-actin
- Calpain
- Cofilin1
- Epithelial-mesenchymal transition
- Ezrin
- ITRAQ
- Lung cancer
- Moesin
- Qualitative proteomics
- TGF-β