The Arabidopsis thaliana ribosomal protein S15 (rig) gene

Veena Sangwan, Todd R. Lenvik, J. Stephen Gantt

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We have isolated cDNA and genomic clones for Arabidopsis thaliana cytosolic ribosomal protein S15 and determined their sequences. Like animal S15 genes, this plant S15 gene is composed of four exons and the first intron is located immediately following the ATG translational start codon. The 5′ end of the S15 mRNA was mapped by RNase protection experiments which showed that this mRNA contains a 5′ untranslated region of approx. 83 nucleotides. Southern blot analyses suggest that Arabidopsis S15 is encoded by a small family of genes. The sequences of the predicted exons in the cloned S15 gene are identical to that of the S15 cDNA, demonstrating that this gene is transcriptionally active. Sequence analysis of the cloned A. thaliana S15 gene shows that it is tightly linked (approx. 500 nucleotides distant) to a gene of unknown function. The Arabidopsis S15 protein described here is about 75% identical to vertebrate S15, 70% identical to the homologous yeast protein (S21), 50% identical to archaebacterial S19, 30% identical to eubacterial S19, and about 30% identical to plant mitochondrial and plastid S19.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalBBA - Gene Structure and Expression
Issue number2
StatePublished - Nov 16 1993

Bibliographical note

Funding Information:
We thank Michael Thompson, Sudam Pathirana, and Jocelyn Shaw for suggestions during the preparation of this manuscript. This work was supported by a grant from the Public Health Service National Institutes of Health (GM-38769).


  • (A. thaliana)
  • Ribosomal protein gene
  • cDNA
  • mRNA


Dive into the research topics of 'The Arabidopsis thaliana ribosomal protein S15 (rig) gene'. Together they form a unique fingerprint.

Cite this