The cell-surface proteoglycan Dally regulates Wingless signalling in Drosophila

Manabu Tsuda, Keisuke Kamimura, Hiroshi Nakato, Michael Archer, William Staatz, Bethany Fox, Melanie Humphrey, Sara Olson, Tracy Futch, Vesna Kaluza, Esther Siegfried, Lynn Stam, Scott B. Selleck

Research output: Contribution to journalArticlepeer-review

342 Scopus citations

Abstract

Wingless (Wg) is a member of the Wnt family of growth factors, secreted proteins that control proliferation and differentiation during development. Studies in Drosophila have shown that responses to Wg require cell-surface heparan sulphate, a glycosaminoglycan component of proteoglycans. These findings suggest that a cell-surface proteoglycan is a component of a Wg/Wnt receptor complex. We demonstrate here that the protein encoded by the division abnormally delayed (dally) gene is a cell-surface, heparan-sulphate- modified proteoglycan, dally partial loss-of-function mutations compromise Wg-directed events, and disruption of dally function with RNA interference produces phenotypes comparable to those found with RNA interference of wg or frizzled (fz)/Dfz2 (ref. 7). Ectopic expression of Dally potentiates Wg signalling without altering levels of Wg and can rescue a wg partial loss- of-function mutant. We also show that dally, a regulator of Decapentaplegic (Dpp) signalling during post-embryonic development, has tissue-specific effects on Wg and Dpp signalling. Daily can therefore differentially influence signalling mediated by two growth factors, and may form a regulatory component of both Wg and Dpp receptor complexes.

Original languageEnglish (US)
Pages (from-to)276-280
Number of pages5
JournalNature
Volume400
Issue number6741
DOIs
StatePublished - Jul 15 1999

Fingerprint Dive into the research topics of 'The cell-surface proteoglycan Dally regulates Wingless signalling in Drosophila'. Together they form a unique fingerprint.

Cite this