The cellular origin of laminin determines its role in blood pressure regulation

Yao Yao, Erin H. Norris, Sidney Strickland

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Laminin of different cellular sources has distinct functions. In addition to vascular smooth muscle cells (SMCs), aorta also contains a small population of nestin? cells, whose function remains unknown. This study investigates the role of SMC- and nestin? cell-derived laminin in blood pressure (BP) regulation and SMC contractibility. Using mice with laminin deficiency in SMCs (SKO) or nestin? cells (NKO), we examined laminindependent changes in BP. Contractile protein expression was reduced in SKO but not NKO mice, consistent with their, respectively, low and normal baseline BP measurements. At the ultrastructural level, SKO SMCs maintained the contractile phenotype with reduced elasticity, whereas NKO SMCs switched to the synthetic phenotype and showed degeneration. Additionally, angiotensin II (Ang II) significantly increased BP in SKO but not NKO mice. It also enhanced contractile proteins to the same levels and induced SMC degeneration in both knockout mice. These data suggest that SMC laminin regulates BP via modulating contractile protein expression, whereas nestin? cellderived laminin contributes to SMC phenotypic switch.

Original languageEnglish (US)
Pages (from-to)999-1008
Number of pages10
JournalCellular and Molecular Life Sciences
Volume72
Issue number5
DOIs
StatePublished - Mar 1 2015

Keywords

  • Aorta
  • Laminin
  • Nestin
  • Smooth muscle cells

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