The chloroform fraction of Carpinus tschonoskii leaves inhibits the production of inflammatory mediators in HaCaT keratinocytes and RAW264.7 macrophages

Gyeoung Jin Kang, Na Jin Kang, Sang Chul Han, Dong Hwan Koo, Hee Kyoung Kang, Byoung Sam Yoo, Eun Sook Yoo

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Inflammation is the immune system's response to infection and injury-related disorders, and is related to pro-inflammatory factors (NO, PGE2, cytokines, etc.) produced by inflammatory cells. Atopic dermatitis (AD) is a representative inflammatory skin disease that is characterized by increasing serum levels of inflammatory chemokines, including macrophage-derived chemokine (MDC). Carpinus tschonoskii is a member of the genus Carpinus. We investigated the anti-inflammatory activity of C. tschonoskii by studying the effects of various solvent fractions prepared from its leaves on inflammatory mediators in HaCaT and RAW264.7 cells. We found that the chloroform fraction of C. tschonoskii inhibited MDC at both the protein and mRNA levels in HaCaT cells, acting via the inhibition of STAT1 in the IFN-γ signaling pathway. In addition, the chloroform fraction significantly suppressed the expression of inflammatory factors induced by lipopolysaccharide stimulation, except COX-2 and TNF-α. These results suggest that the chloroform fraction of C. tschonoskii leaves may include a component with potential anti-inflammatory activity.

Original languageEnglish (US)
Pages (from-to)255-262
Number of pages8
JournalToxicological Research
Volume28
Issue number4
DOIs
StatePublished - Dec 2012

Keywords

  • Carpinus tschonoskii
  • Hacat keratinocytes
  • Inflammation mediators
  • RAW264.7 macrophages

Fingerprint Dive into the research topics of 'The chloroform fraction of Carpinus tschonoskii leaves inhibits the production of inflammatory mediators in HaCaT keratinocytes and RAW264.7 macrophages'. Together they form a unique fingerprint.

Cite this