Abstract
A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.
Original language | English (US) |
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Pages (from-to) | 40-43 |
Number of pages | 4 |
Journal | Diagnostic Microbiology and Infectious Disease |
Volume | 89 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2017 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2017 Elsevier Inc.
Keywords
- Aberrant real-time PCR
- Adenovirus
- Reduced fluorescence