A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.
|Original language||English (US)|
|Number of pages||4|
|Journal||Diagnostic Microbiology and Infectious Disease|
|State||Published - Sep 2017|
- Aberrant real-time PCR
- Reduced fluorescence