TY - JOUR
T1 - The effect of a hyaluronan-carboxymethylcellulose membrane vs. polyglactin 910 mesh on intra-abdominal tumor formation in mice
AU - Lee, Peter K.
AU - Windsperger, Andrew P.
AU - Wilson, Christopher M.
AU - McCarthy, James B.
AU - Wasiluk, Karen R.
AU - Rothenberger, David A.
AU - Bullard Dunn, Kelli M.
PY - 2008/9
Y1 - 2008/9
N2 - PURPOSE: Hyaluronan mediates growth of SW620 colon cancer cells. Because hyaluronan is the active ingredient in Seprafilm®, we hypothesized that Seprafilm® would affect intraperitoneal tumor growth in a mouse model of peritoneal seeding. METHODS: Immunodeficient mice underwent laparotomy and intraperitoneal inoculation of 105 SW620 cells. Seprafilm® (n = 22), Vicryl mesh (foreign body control; n = 24), or no material (sham; n = 19) was placed under the incision. Mice were killed after four weeks and tumors were dissected, counted, and weighed. RESULTS: Ninety-five percent of mice in the sham group and 96 percent in the Vicryl group developed intraperitoneal tumors. In contrast, only 64 percent of mice in the Seprafilm® group developed tumors (P = 0.024), and these tumors were smaller than those in the sham group; (Seprafilm® = 42 ± 9 mg vs. sham = 82 ± 17 mg; P = 0.05). In contrast, tumors in the Vicryl group were dramatically larger (349 ± 49 mg; P < 0.001 vs. sham or Seprafilm®). CONCLUSIONS: Despite previous data that suggested that hyaluronan increases colon cancer cell growth, we found that Seprafilm® decreased tumor formation and tended to decrease size in this model. In contrast, Vicryl mesh increased tumor formation and size. Our results suggest that Seprafilm® does not promote intraperitoneal tumor growth, especially compared with Vicryl mesh.
AB - PURPOSE: Hyaluronan mediates growth of SW620 colon cancer cells. Because hyaluronan is the active ingredient in Seprafilm®, we hypothesized that Seprafilm® would affect intraperitoneal tumor growth in a mouse model of peritoneal seeding. METHODS: Immunodeficient mice underwent laparotomy and intraperitoneal inoculation of 105 SW620 cells. Seprafilm® (n = 22), Vicryl mesh (foreign body control; n = 24), or no material (sham; n = 19) was placed under the incision. Mice were killed after four weeks and tumors were dissected, counted, and weighed. RESULTS: Ninety-five percent of mice in the sham group and 96 percent in the Vicryl group developed intraperitoneal tumors. In contrast, only 64 percent of mice in the Seprafilm® group developed tumors (P = 0.024), and these tumors were smaller than those in the sham group; (Seprafilm® = 42 ± 9 mg vs. sham = 82 ± 17 mg; P = 0.05). In contrast, tumors in the Vicryl group were dramatically larger (349 ± 49 mg; P < 0.001 vs. sham or Seprafilm®). CONCLUSIONS: Despite previous data that suggested that hyaluronan increases colon cancer cell growth, we found that Seprafilm® decreased tumor formation and tended to decrease size in this model. In contrast, Vicryl mesh increased tumor formation and size. Our results suggest that Seprafilm® does not promote intraperitoneal tumor growth, especially compared with Vicryl mesh.
KW - Colon cancer
KW - Hyaluronan
KW - Polyglactin
KW - Seprafilm®
KW - Vicryl
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U2 - 10.1007/s10350-008-9299-z
DO - 10.1007/s10350-008-9299-z
M3 - Article
C2 - 18418651
AN - SCOPUS:49749118658
SN - 0012-3706
VL - 51
SP - 1403
EP - 1407
JO - Diseases of the colon and rectum
JF - Diseases of the colon and rectum
IS - 9
ER -