The effect of bupivacaine·HCl on the physical properties of neuronal membranes

K. I. Koo, J. H. Bae, C. H. Lee, C. D. Yoon, J. H. Pyun, S. H. Shin, Y. C. Jeon, M. K. Bae, H. O. Jang, W. G. Wood, I. Yun

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Fluorescent probe techniques were used to evaluate the effect of bupivacaine·HCl on the physical properties (transbilayer asymmetric lateral and rotational mobilities, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. An experimental procedure was used based on selective quenching of both 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5- hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer (RET) from the tryptophans of membrane proteins to Py-3-Py. Bupivacaine· HCl increased the bulk lateral and rotational mobilities, and annular lipid fluidity in SPMVs lipid bilayers, and had a greater fluidizing effect on the inner monolayer than that of the outer monolayer. The magnitude of increasing effect on annular lipid fluidity in SPMVs lipid bilayer induced by bupivacaine·HCl was significantly far greater than magnitude of increasing effect of the drug on the lateral and rotational mobilities of bulk SPMVs lipid bilayer. It also caused membrane proteins to cluster. These effects of bupivacaine·HCl on neuronal membranes may be responsible for some, though not all, of the local anesthetic actions of bupivacaine·HCl.

Original languageEnglish (US)
Pages (from-to)3-12
Number of pages10
JournalProtoplasma
Volume234
Issue number1-4
DOIs
StatePublished - Dec 1 2008

Keywords

  • Annular lipid fluidity
  • Bupivacaine·HCl
  • Membrane protein clustering
  • Neuronal membranes
  • Transbilayer lateral and rotational mobilities

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