The nucleic acid stains DAPI and SYTO-13 were used to stain particulate organic matter (POM) samples from the lower Chesapeake Bay and from a culture of Synechococcus (CCMP 837). Cell counts determined using each stain were compared as was the effect of each stain on the organic matter composition of the POM. Both stains yield similar cell counts, though SYTO-13 counts are lower than DAPI counts for each Chesapeake Bay sample site. The stains do not significantly affect organic matter composition as determined using direct temperature-resolved mass spectrometry (DT-MS) and pyrolysis-gas chromatography-mass spectrometry (PyGCMS). This means that either stain could be used for cell identification within samples prior to molecular-level characterization of the same samples.
Bibliographical noteFunding Information:
Thanks to Carl Johnson (WHOI) for DT-MS assistance, Martina Doblin (ODU) for advice and assistance in culturing Synechococcus, and Prashanth Nallathamby (ODU) for additional staining/microscopy work. This research was supported by the donors of the Petroleum Research Fund, administered by the ACS, and by an ODU Summer Research Fellowship (to ECM).
Copyright 2018 Elsevier B.V., All rights reserved.
- Mass spectrometry
- Particulate organic matter