The effect of dimethylsulfoxide on the water transport response of rat liver tissue during freezing

David J. Smith, Linda D. Pham, John C. Bischof

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7 Scopus citations

Abstract

This work investigates the biophysical response of water transport in a clinically important tissue type-liver-to freezing in the presence of dimethylsulfoxide (DMSO). Sprague-Dawley rat liver tissue samples were frozen in RPMI media supplemented with 1 and 2 M DMSO at a cooling rate of 5°C/min. The water transport was measured by cell volumetric changes as assessed by low temperature freeze substitution microscopy and image analysis. These data were compared to a previous study where liver in the absence of DMSO was cooled at the same rate and shown to be completely dehydrated at -20°C [27]. Curve-fitting a water transport model to the experimental data showed that L(pg) and E(Lp) drop from 19 μm/min-atm and 69 kcal/mole in the absence of DMSO [27] to 0.033 μm/min-atm and 8.1 kcal/mol in 2 M DMSO. The available data were unable to support a statistically significant difference between the 1 M and 2 M DMSO parameters.

Original languageEnglish (US)
Pages (from-to)343-354
Number of pages12
JournalCryo-Letters
Volume19
Issue number6
StatePublished - Nov 1998

Keywords

  • CPA
  • Cryopreservation
  • Dimethylsulfoxide
  • Freezing
  • Hydraulic permeability
  • Liver
  • Water transport

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