The effect of exhaustive exercise on the antioxidant enzyme system in skeletal muscle from calcium-deficient rats

Shuji Oh-ishi, Takako Kizaki, Tomomi Ookawara, Koji Toshinai, Shukoh Haga, Fujio Karasawa, Tetsuo Satoh, Naokazu Nagata, Li Li Ji, Hideki Ohno

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The aim of the current study was to elucidate the synergism of dietary calcium restriction and exhaustive exercise in the antioxidant enzyme system of rat soleus muscle, and to investigate the involvement of neutrophils in exercise-induced muscle damage. Forty-eight male Wistar rats were assigned to the following groups: control (C) or calcium-restricted [1 month (1 M) or 3 months (3 M)]. Each group was subdivided into acutely exercised or non- exercised groups. Soleus muscle from each rat was analysed to determine the levels of antioxidant enzymes [Mn-superoxide dismutase (SOD), Cu,Zn-SOD, glutathione peroxidase (GPX), and catalase (CAT)]. Dietary calcium restriction resulted in calcium deficiency and upregulated the antioxidant enzymes examined except GPX. Conversely, exhaustive exercise significantly decreased GPX and CAT, but not SODs activities in the calcium-restricted (1 M and/or 3 M) rats. Contents of immunoreactive Mn-SOD and Cu,Zn-SOD were only increased in the 3 M rats. During calcium restriction, the mRNA expression of both forms of SOD showed initial upregulation, followed by downregulation. Exhaustive exercise significantly increased the mRNA expressions only in the 3 M rats. Moreover, exhaustive exercise markedly increased myeloperoxidase activity in soleus muscles from the 1 M and 3 M rats compared with the C rats, and significantly enhanced the ability of neutrophils to generate superoxide in the 3 M rats. The results demonstrate that dietary calcium restriction upregulates certain antioxidant enzyme activities in rat soleus muscle, indicating an enhanced resistance to potential increases in intracellular reactive oxygen species. The results also suggest that exhaustive exercise may cause oxidative damage in soleus muscle of calcium- deficient rats through the activation of neutrophils.

Original languageEnglish (US)
Pages (from-to)767-774
Number of pages8
JournalPflugers Archiv European Journal of Physiology
Volume435
Issue number6
DOIs
StatePublished - May 7 1998

Keywords

  • Catalase
  • Dietary calcium restriction
  • Glutathione peroxidase
  • Myeloperoxidase
  • Neutrophil
  • Soleus muscle
  • Superoxide
  • Superoxide dismutase

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