THE EFFECT OF MORPHINE TOLERANCE and DEPENDENCE ON CELL FREE PROTEIN SYNTHESIS

F. Rederick, B. Craves, Horace H Loh, James L. Meyerhoff

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A cell free system consisting of polyribosomes and pH 5 factors of the cytosol was isolated from mouse brain. This system actively promoted the incorporation of radiolabeled amino acids into protein in vitro. Addition of exogenous morphine to a cell free protein synthetic system isolated from chronically morphinized, placebo treated, or naive mouse brain had no effect on the relative synthetic capacity of the system. In addition, morphine did not alter the response to a synthetic mRNA, polyuridylic acid. However, both the polyribosomes and pH 5 factors isolated from chronically morphinized mouse brain were more effective in promoting amino acid incorporation into protein relative to the corresponding fractions from placebo treated mice. Acrylamide gel electrophoresis of the proteins in the incubation mixture showed the increased amino acid incorporation was the result of a general quantitative increase in the specific activity of all of the proteins synthesized by the cell free system.

Original languageEnglish (US)
Pages (from-to)1309-1316
Number of pages8
JournalJournal of Neurochemistry
Volume31
Issue number5
DOIs
StatePublished - Nov 1978
Externally publishedYes

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