The effect of purification method on the completeness of the immature HIV-1 Gag shell

Nitzan Kol; Marianna Tsvitov; Liron Hevroni; Sharon G. Wolf; Hong Bo Pang; Michael S. Kay; Itay Rousso

doi:10.1016/j.jviromet.2010.07.035

The effect of purification method on the completeness of the immature HIV-1 Gag shell

Nitzan Kol, Marianna Tsvitov, Liron Hevroni, Sharon G. Wolf, Hong Bo Pang, Michael S. Kay, Itay Rousso

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Elucidating the structure of the immature HIV-1 Gag core is an important aspect of understanding the biology of this virus. In doing so, preservation of the fragile Gag lattice is essential. In this study, the effects of purification methods on the structural and mechanical integrity of immature HIV-1 are examined. The results show that the morphological and mechanical properties of the virion are preserved to a significantly higher degree by Iodixanol (OptiPrep) purification compared to the standard sucrose method. In conclusion, these results indicate that OptiPrep instead of sucrose purification should be employed when conducting structural studies on the HIV-1 virion.

Original language	English (US)
Pages (from-to)	244-247
Number of pages	4
Journal	Journal of Virological Methods
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2010.07.035
State	Published - Oct 2010
Externally published	Yes

Bibliographical note

Funding Information:
We thank Alan Rein for helpful discussions and comments on this work. This work was supported in part by NIH grant AI076735 to I.R. and M.K. I.R. holds the Robert Edwards and Roselyn Rich Manson Career Development Chair. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science.

Keywords

Gag
HIV-1
Immature
Iodixanol
OptiPrep
Purification
Structure
Sucrose

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "The effect of purification method on the completeness of the immature HIV-1 Gag shell",

abstract = "Elucidating the structure of the immature HIV-1 Gag core is an important aspect of understanding the biology of this virus. In doing so, preservation of the fragile Gag lattice is essential. In this study, the effects of purification methods on the structural and mechanical integrity of immature HIV-1 are examined. The results show that the morphological and mechanical properties of the virion are preserved to a significantly higher degree by Iodixanol (OptiPrep) purification compared to the standard sucrose method. In conclusion, these results indicate that OptiPrep instead of sucrose purification should be employed when conducting structural studies on the HIV-1 virion.",

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note = "Funding Information: We thank Alan Rein for helpful discussions and comments on this work. This work was supported in part by NIH grant AI076735 to I.R. and M.K. I.R. holds the Robert Edwards and Roselyn Rich Manson Career Development Chair. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science.",

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AU - Hevroni, Liron

AU - Wolf, Sharon G.

AU - Pang, Hong Bo

AU - Kay, Michael S.

AU - Rousso, Itay

N1 - Funding Information: We thank Alan Rein for helpful discussions and comments on this work. This work was supported in part by NIH grant AI076735 to I.R. and M.K. I.R. holds the Robert Edwards and Roselyn Rich Manson Career Development Chair. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science.

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AB - Elucidating the structure of the immature HIV-1 Gag core is an important aspect of understanding the biology of this virus. In doing so, preservation of the fragile Gag lattice is essential. In this study, the effects of purification methods on the structural and mechanical integrity of immature HIV-1 are examined. The results show that the morphological and mechanical properties of the virion are preserved to a significantly higher degree by Iodixanol (OptiPrep) purification compared to the standard sucrose method. In conclusion, these results indicate that OptiPrep instead of sucrose purification should be employed when conducting structural studies on the HIV-1 virion.

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KW - Structure

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The effect of purification method on the completeness of the immature HIV-1 Gag shell

Abstract

Bibliographical note

Keywords

UN SDGs

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