The effect of transforming growth factor-β1 on glycosaminoglycan production by human marrow cultures

D. L. Uhlman, Daniel L Mooradian, Leo T Furcht, Sharon D Luikart

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We have assayed the effect of transforming growth factor-β1 (TGF-β1), a potent modulator of hematopoiesis, on glycosaminoglycan production in human marrow cultures. Glycosaminoglycans are a component of the extracellular matrix known to affect cell growth and differentiation. TGF-β1 and [35S]sulfate were added simultaneously to hematopoietically active human marrow cultures, and radiolabeled glycosaminoglycan production was determined by cetylpyridinium chloride precipitation. TGF-β1 at 15 ng/ml for 72 h increased [35S]sulfate incorporation into media glycosaminoglycans to 190% of control levels but did not affect the [35S]sulfate incorporation into cell-associated glycosaminoglycans. Approximately 90% of the glycosaminoglycans in the media fraction and 85% of the glycosaminoglycans in the cell-associated fraction were susceptible to degradation by chrondroitin ABCase in both treated and control cultures. Pulse-chase experiments suggested that the increase in glycosaminoglycan [35S]sulfate incorporation was not due to decreased glycosaminoglycan degradation. This concentration of TGF-β1 did not alter nonadherent granulocyte-macrophage colony-forming unit (CFU-GM) number per flask but significantly decreased the more primitive adherent CFU-GM number per flask (by 50%-70%). These data suggest that the ability of TGF-β1 to modulate hematopoiesis may be due, in part, to its effects on glycosaminoglycan production.

Original languageEnglish (US)
Pages (from-to)1121-1125
Number of pages5
JournalExperimental Hematology
Volume18
Issue number10
StatePublished - 1990

Keywords

  • Glycosaminoglycans
  • TGF-β
  • extracellular matrix
  • hematopoiesis

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