The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product

S. K. Armstrong, G. S. Pettis, L. J. Forrester, M. A. Mclntosh

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The nucleotide sequence of the Escherichia coli enterobactin biosynthesis gene entD has been determined. entD specifies a predicted 23579 Dalton protein containing several helical regions, a trans‐membrane segment and one positively charged domain. The EntD polypeptide was overexpressed and identified in electrophoretic gels as a membrane protein. Although results of conventional membrane fractionation techniques were inconclusive, protease accessibility studies provided evidence that EntD domains are exposed on the inner leaflet of the cytoplasmic membrane. The presence of repetitive extragenic palindromic (REP) sequences within the fepA‐entD intercistronic region was confirmed. Lack of a canonical promoter and an iron control region 5’to entD, along with RNA hybridization data, suggest that an iron‐regulated transcript contains both fepA and entD.

Original languageEnglish (US)
Pages (from-to)757-766
Number of pages10
JournalMolecular Microbiology
Volume3
Issue number6
DOIs
StatePublished - Jun 1989

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