When the 16S rDNA of predominant Escherichia coli strains from cattle was digested with HhaI and HaeIII, the strains could be sub-divided into four operational taxonomic units. When genomic DNA was digested with XbaI, strains could be grouped into 24 pulsed-field gel electrophoresis genotypes (>95% Dice similarity) and five clades (>20% Dice similarity). Diet (hay versus grain) and gastrointestinal compartment (rumen versus colon) did not have a large impact on diversity. However, both analyses indicated that the cows (n=2) had different E. coli populations. When all 22 colonic strains were inoculated into a maltose-limited chemostat, only a single genotype persisted. Based on these results, the genetic diversity of E. coli in the cattle is very great and this bacterium can occupy different niches. Copyright (C) 2000 Federation of European Microbiological Societies.
Bibliographical noteFunding Information:
This work was supported by a grant from the US Food and Drug Administration (FD-U-001576-02). J.B.R. is a member of the US Dairy Forage Research Center, Madison, WI, USA.
- 16S rRNA
- Escherichia coli
- Genetic diversity
- Pulsed-field gel electrophoresis