TY - JOUR
T1 - The identification and quantitation of alkylated nucleobases by high-performance liquid chromatograghy with UV photodiode array detection
AU - Xue, W.
AU - Samanta, A. K.
AU - Carlson, Robert M
PY - 1990/1/1
Y1 - 1990/1/1
N2 - The application of UV diode array detection in high-performance liquid chromatographic (HPLC) identification and quantitation of several classes of synthetic and commercially available alkylated nucleobases is investigated. Quantitative spectral overlays of these compounds to methyl standard references from a spectral library and absorbance ratios at two maximal wavelengths (λ(max)) are found to be useful in categorizing the solutes. They can be grouped into classes of compounds originating from a specific nucleobase and classes of analogs having different alkyl substituents (e.g., methyl, ethyl, propyl, allyl, and benzyl) at the same position of the heterocycle. At a selected wavelength for alkylated nucleobases in the same class, the detector response factors are independent of the alkyl group (±10%). This technique provides a practical means for both qualitative and quantitative analysis of product distribution of DNA base alkylation by using only readily obtainable methylated derivatives as the reference standards.
AB - The application of UV diode array detection in high-performance liquid chromatographic (HPLC) identification and quantitation of several classes of synthetic and commercially available alkylated nucleobases is investigated. Quantitative spectral overlays of these compounds to methyl standard references from a spectral library and absorbance ratios at two maximal wavelengths (λ(max)) are found to be useful in categorizing the solutes. They can be grouped into classes of compounds originating from a specific nucleobase and classes of analogs having different alkyl substituents (e.g., methyl, ethyl, propyl, allyl, and benzyl) at the same position of the heterocycle. At a selected wavelength for alkylated nucleobases in the same class, the detector response factors are independent of the alkyl group (±10%). This technique provides a practical means for both qualitative and quantitative analysis of product distribution of DNA base alkylation by using only readily obtainable methylated derivatives as the reference standards.
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U2 - 10.1093/chromsci/28.9.477
DO - 10.1093/chromsci/28.9.477
M3 - Article
C2 - 2273070
AN - SCOPUS:0025483743
SN - 0021-9665
VL - 28
SP - 477
EP - 481
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 9
ER -