The influence of a spatiotemporal 3D environment on endothelial cell differentiation of human induced pluripotent stem cells

Sophia Zhang, James R. Dutton, Liping Su, Jianyi Zhang, Lei Ye

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Current EC differentiation protocols are inefficient, and the phenotypes of the differentiated ECs are only briefly stable, which significantly inhibits their utility for basic science research. Here, a remarkably more efficient hiPSC-EC differentiation protocol that incorporates a three-dimensional (3D) fibrin scaffold is presented. With this protocol, up to 45% of the differentiated hiPSCs assumed an EC phenotype, and after purification, greater than 95% of the cells displayed the EC phenotype (based on CD31 expression). The hiPSC-ECs continued to display EC characteristics for 4 weeks invitro. Gene and protein expression levels of CD31, CD144 and von Willebrand factor-8 (vWF-8) were significantly up-regulated in differentiated hiPSC-ECs. hiPSC-ECs also have biological function to up-take Dil-conjugated acetylated LDL (Dil-ac-LDL) and form tubular structures on Matrigel. Collectively, these data demonstrate that a 3D differentiation protocol can efficiently generate ECs from hiPSCs and, furthermore, the differentiated hiPSC-ECs are functional and can maintain EC fate up to 4 weeks invitro.

Original languageEnglish (US)
Pages (from-to)3786-3793
Number of pages8
JournalBiomaterials
Volume35
Issue number12
DOIs
StatePublished - Apr 2014

Bibliographical note

Funding Information:
This work was supported by US Public Health Service grants NIH RO1 HL67828 , HL95077 , HL114120 , and UO1HL100407 . The authors would like to thank Mr. W. Kevin Meisner for his editorial assistance.

Keywords

  • Cell differentiation
  • Eddothelial cells
  • Human induced pluripotent stem cells
  • Scaffold

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