The influence of adrenergic and cholinergic agents on the chemiluminescent and mitogenic responses of leukocytes from the rainbow trout, Oncorhyncus mykiss

Craig M. Flory, Christopher J. Bayne

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

In a previous report, it was shown that agonists of cholinergic or alpha-adrenergic receptors enhance, whereas beta-adrenergic agonists suppress, in vitro antibody responses by splenic leukocytes from rainbow trout. The present study addresses the mechanisms by which autonomic neurotransmitters (or their analogs) could affect this antibody response. Possibilities include an influence on accessory cell function, on the clonal proliferation of antigen-stimulated lymphocytes, and/or on the synthesis and secretion of antibody. Epinephrine and selective beta-adrenergic agonists suppressed whereas both alpha and cholinergic receptor agonists enhanced the ability of stimulated pronephric leukocytes (mainly macrophages and neutrophils) to produce reactive oxygen species, suggesting that accessory cells may be a target of these agents in the antibody response. Beta-adrenergic agonists also suppressed the proliferative response of splenic leukocytes to LPS, Con A, and PHA. There was no effect, however, of alpha-adrenergic or cholinergic-receptor agonists on mitogenic responses.

Original languageEnglish (US)
Pages (from-to)135-142
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume15
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

Bibliographical note

Funding Information:
Address correspondence to Craig M. Flory, at The Trudeau Institute, P.O. Box 59, Saranac Lake, NY, 12983. This work was supported in part by research funds from the Department of Zoology, Oregon State University, and in part by a National Institute of Mental Health Predoctoral Fellowship.

Keywords

  • Accessory cells
  • Chemiluminescence
  • Immunopharmacology
  • Immunoregulation
  • Mitogenesis
  • Neuroimmunology
  • Neurotransmitters
  • Rainbow trout
  • Respiratory burst

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