The potential of RT-PCR to rapidly diagnose dengue infections from both acute and convalescent phase patients' sera was evaluated. The RNA extraction method involved binding of the viral RNA to silica particles in the presence of high concentration of guanidine thiocyanate. The protocol that was established was sensitive enough to detect 40 plaque forming units per 100 microliter of serum and results could be obtained within one day. Results from this study indicate that clinical samples should be collected in the early acute phase of illness when anti-dengue antibodies were undetectable or of low titres to ensure a more reliable diagnosis.
Bibliographical noteFunding Information:
This project was partially sponsored by the Ministry of Science, Technology and Environment, Malaysia; the W.H.O. Regional Office for the Western Pacific, Philippines, and the University of Malaya, Malaysia. Cloned, serotype-specificp robes were kindly provided by Dr. V. Deubel, Institut Pasteur,P aris, France.
- Anti-dengue antibody
- Dengue diagnosis