The magnetic susceptibility of reduced cytochrome P-450cam

P. M. Champion; E. Münck; P. G. Debrunner; T. H. Moss; J. D. Lipscomb; I. C. Gunsalus

doi:10.1016/0005-2728(75)90179-6

The magnetic susceptibility of reduced cytochrome P-450_cam

P. M. Champion, E. Münck, P. G. Debrunner, T. H. Moss, J. D. Lipscomb, I. C. Gunsalus

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The magnetic susceptibility of singly reduced, camphor-complexed cytochrome P-450 from Pseudomonas putida has been measured at various temperatures. Computer analysis using a spin Hamiltonian gives values of D between 15K and 25K, which agrees with a value of D = 20K found by low-field variable temperature Mössbauer spectroscopy. This result is compared with those from deoxyhemoglobin and deoxy-myoglobin.

Original language	English (US)
Pages (from-to)	579-582
Number of pages	4
Journal	BBA - Bioenergetics
Volume	376
Issue number	3
DOIs	https://doi.org/10.1016/0005-2728(75)90179-6
State	Published - Mar 20 1975

Bibliographical note

Funding Information:
high-spin iron atom in reduced P-450 has an isolated ground state oribital (with spin quintet) conducive to the spin Hamiltonian formalism, while the heme iron of deoxyhemoglobin and deoxymyoglobin might be better described Using a different approach \[1 2 \]. The authors would like to thank M.J. Na~ntvedt for laboratory assistance. This work was partially supported hy U.S. Public Health Service grant GM 16406

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title = "The magnetic susceptibility of reduced cytochrome P-450cam",

abstract = "The magnetic susceptibility of singly reduced, camphor-complexed cytochrome P-450 from Pseudomonas putida has been measured at various temperatures. Computer analysis using a spin Hamiltonian gives values of D between 15K and 25K, which agrees with a value of D = 20K found by low-field variable temperature M{\"o}ssbauer spectroscopy. This result is compared with those from deoxyhemoglobin and deoxy-myoglobin.",

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AU - Champion, P. M.

AU - Münck, E.

AU - Debrunner, P. G.

AU - Moss, T. H.

AU - Lipscomb, J. D.

AU - Gunsalus, I. C.

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