The mec-8 gene of C. Elegans encodes a protein with two RNA recognition motifs and regulates alternative splicing of unc-52 transcripts

Erik A. Lundquist, Robert K. Herman, Teresa M. Rogalski, Gregory P. Mullen, Donald G. Moerman, Jocelyn E. Shaw

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Mutations in the mec-8 gene of Caenorhabditis elegans were previously shown to affect the functions of body wall muscle and mechanosensory and chemosensory neurons, Mutations in mec-8 also strongly enhance the mutant phenotype of specific mutations in unc-52, a gene that encodes, via alternative splicing of pre-mRNA, a set of basement membrane proteins, homologs of perlecan, that are important for body wall muscle assembly and attachment to basement membrane, hypodermis and cuticle, We have cloned mec-8 and found that it encodes a protein with two RNA recognition motifs, binding proteins, We have used reverse transcription-PCR and RNase protection experiments to show that mec-8 regulates the accumulation of a specific subset of alternatively spliced unc-52 transcripts, We have also shown with antibodies to UNC-52 that mec-8 affects the abundance of a subset of UNC-52 isoforms. We propose that mec-8 encodes a trans-acting factor that regulates the alternative splicing of the pre-mRNA of unc-52 and one or more additional genes that affect mechanosensory and chemosensory neuron function.

Original languageEnglish (US)
Pages (from-to)1601-1610
Number of pages10
JournalDevelopment
Volume122
Issue number5
StatePublished - Jun 5 1996

Keywords

  • Alternative RNA splicing
  • C. elegans
  • Mec-8 gene
  • RRMS
  • Unc-52 gene

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