The mesenchymoangioblast, mesodermal precursor for mesenchymal and endothelial cells

Igor I. Slukvin, Akhilesh Kumar

Research output: Contribution to journalReview articlepeer-review

Abstract

Mesenchymoangioblast (MB) is the earliest precursor for endothelial and mesenchymal cells originating from APLNR + PDGFRα + KDR + mesoderm in human pluripotent stem cell cultures. MBs are identified based on their capacity to form FGF2-dependent compact spheroid colonies in a serum-free semisolid medium. MBs colonies are composed of PDGFRβ + CD271 + EMCN + DLK1 + CD73 primitive mesenchymal cells which are generated through endothelial/angioblastic intermediates (cores) formed during first 3–4 days of clonogenic cultures. MB-derived primitive mesenchymal cells have potential to differentiate into mesenchymal stromal/stem cells (MSCs), pericytes, and smooth muscle cells. In this review, we summarize the specification and developmental potential of MBs, emphasize features that distinguish MBs from other mesenchymal progenitors described in the literature and discuss the value of these findings for identifying molecular pathways leading to MSC and vasculogenic cell specification, and developing cellular therapies using MB-derived progeny.

Original languageEnglish (US)
Pages (from-to)3507-3520
Number of pages14
JournalCellular and Molecular Life Sciences
Volume75
Issue number19
DOIs
StatePublished - Oct 1 2018
Externally publishedYes

Bibliographical note

Funding Information:
We thank Matthew Raymond for editorial assistance. I.I.S. and A.K are supported by funds from the National Institute of Health (U01HL134655, U01HL099773 and P51 RR000167). I.I.S. is a founding shareholder and consultant for Cynata Therapeutics.

Publisher Copyright:
© 2018, Springer Nature Switzerland AG, part of Springer Nature.

Keywords

  • Cardiovascular progenitors
  • Embryonic mesenchyme
  • Embryonic stem cells
  • Hemangioblasts
  • Human pluripotent stem cells
  • Induced pluripotent stem cells
  • Mesenchymal stem cells
  • Mesenchymoangioblasts
  • Mesoangioblast
  • Mesoderm development
  • Mesospheres
  • Pericytes
  • Smooth muscles

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