The methylation process in corrin biosynthesis. Application of 1H{13C} nuclear magnetic resonance difference spectroscopy to a biochemical problem

A. Ian Scott, Masahiro Kajiwara, Tamiko Takahashi, Ian M. Armitage, Peter Demou, David Petrocine

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19 Scopus citations

Abstract

1H{13C} n.m.r. difference spectroscopy has been used to determine the intact incorporation of [methyl-13CD 3]methionine into the C-1- and C-12α-methyl groups of vitamin B12.

Original languageEnglish (US)
Pages (from-to)544-546
Number of pages3
JournalJournal of the Chemical Society, Chemical Communications
Issue number14
DOIs
StatePublished - 1976

Bibliographical note

Funding Information:
COMM., 1976 methyl groups and the proton mobility at C-18 and C-19, as well as the nature of the liberated C-20 carbon. Using different techniques, Professors Arigoni and Battersby have reached identical conclusions regarding the intact nature of C-1 methyl group in corrin biosynthesis as described in the accompanying communications.* We thank the National Institutes of Health and the National Science Foundation for generous support of this research, Dr. J. H. Prestegard for helpful discussions, and Mr. Arnold Brown for cultivating P. sherrnanii. (Received, 21st Afivil 1976; Corn. 447.) -f This value for 3J(C-H) was obtained by computer simulation 1 A. I. Scott, Tetrahedron, 1975, 31, 2639; see also A. R. Battersby, M. Ihara, E. McDonald, F.

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