The methylation process in corrin biosynthesis. Application of 1H{13C} nuclear magnetic resonance difference spectroscopy to a biochemical problem

A. Ian Scott; Masahiro Kajiwara; Tamiko Takahashi; Ian M. Armitage; Peter Demou; David Petrocine

doi:10.1039/C39760000544

The methylation process in corrin biosynthesis. Application of ¹H{¹³C} nuclear magnetic resonance difference spectroscopy to a biochemical problem

A. Ian Scott, Masahiro Kajiwara, Tamiko Takahashi, Ian M. Armitage, Peter Demou, David Petrocine

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

¹H{¹³C} n.m.r. difference spectroscopy has been used to determine the intact incorporation of [methyl-¹³CD ₃]methionine into the C-1- and C-12α-methyl groups of vitamin B₁₂.

Original language	English (US)
Pages (from-to)	544-546
Number of pages	3
Journal	Journal of the Chemical Society, Chemical Communications
Issue number	14
DOIs	https://doi.org/10.1039/C39760000544
State	Published - 1976

Bibliographical note

Funding Information:
COMM., 1976 methyl groups and the proton mobility at C-18 and C-19, as well as the nature of the liberated C-20 carbon. Using different techniques, Professors Arigoni and Battersby have reached identical conclusions regarding the intact nature of C-1 methyl group in corrin biosynthesis as described in the accompanying communications.* We thank the National Institutes of Health and the National Science Foundation for generous support of this research, Dr. J. H. Prestegard for helpful discussions, and Mr. Arnold Brown for cultivating P. sherrnanii. (Received, 21st Afivil 1976; Corn. 447.) -f This value for 3J(C-H) was obtained by computer simulation 1 A. I. Scott, Tetrahedron, 1975, 31, 2639; see also A. R. Battersby, M. Ihara, E. McDonald, F.

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The methylation process in corrin biosynthesis. Application of ¹H{¹³C} nuclear magnetic resonance difference spectroscopy to a biochemical problem. / Scott, A. Ian; Kajiwara, Masahiro; Takahashi, Tamiko; Armitage, Ian M.; Demou, Peter; Petrocine, David.

In: Journal of the Chemical Society, Chemical Communications, No. 14, 1976, p. 544-546.

Research output: Contribution to journal › Article › peer-review

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title = "The methylation process in corrin biosynthesis. Application of 1H{13C} nuclear magnetic resonance difference spectroscopy to a biochemical problem",

abstract = "1H{13C} n.m.r. difference spectroscopy has been used to determine the intact incorporation of [methyl-13CD 3]methionine into the C-1- and C-12α-methyl groups of vitamin B12.",

author = "Scott, {A. Ian} and Masahiro Kajiwara and Tamiko Takahashi and Armitage, {Ian M.} and Peter Demou and David Petrocine",

note = "Funding Information: COMM., 1976 methyl groups and the proton mobility at C-18 and C-19, as well as the nature of the liberated C-20 carbon. Using different techniques, Professors Arigoni and Battersby have reached identical conclusions regarding the intact nature of C-1 methyl group in corrin biosynthesis as described in the accompanying communications.* We thank the National Institutes of Health and the National Science Foundation for generous support of this research, Dr. J. H. Prestegard for helpful discussions, and Mr. Arnold Brown for cultivating P. sherrnanii. (Received, 21st Afivil 1976; Corn. 447.) -f This value for 3J(C-H) was obtained by computer simulation 1 A. I. Scott, Tetrahedron, 1975, 31, 2639; see also A. R. Battersby, M. Ihara, E. McDonald, F. ",

year = "1976",

doi = "10.1039/C39760000544",

language = "English (US)",

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AU - Armitage, Ian M.

AU - Demou, Peter

AU - Petrocine, David

N1 - Funding Information: COMM., 1976 methyl groups and the proton mobility at C-18 and C-19, as well as the nature of the liberated C-20 carbon. Using different techniques, Professors Arigoni and Battersby have reached identical conclusions regarding the intact nature of C-1 methyl group in corrin biosynthesis as described in the accompanying communications.* We thank the National Institutes of Health and the National Science Foundation for generous support of this research, Dr. J. H. Prestegard for helpful discussions, and Mr. Arnold Brown for cultivating P. sherrnanii. (Received, 21st Afivil 1976; Corn. 447.) -f This value for 3J(C-H) was obtained by computer simulation 1 A. I. Scott, Tetrahedron, 1975, 31, 2639; see also A. R. Battersby, M. Ihara, E. McDonald, F.

PY - 1976

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AB - 1H{13C} n.m.r. difference spectroscopy has been used to determine the intact incorporation of [methyl-13CD 3]methionine into the C-1- and C-12α-methyl groups of vitamin B12.

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The methylation process in corrin biosynthesis. Application of ¹H{¹³C} nuclear magnetic resonance difference spectroscopy to a biochemical problem

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