The N-terminal ATPase AT-hook-containing region of the Arabidopsis chromatin-remodeling protein SPLAYED is sufficient for biological activity

Yanhui Su, Chang Seob Kwon, Staver Bezhani, Bärbel Huvermann, Changbin Chen, Angela Peragine, John F. Kennedy, Doris Wagner

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The SNF2-like chromatin-remodeling ATPase SPLAYED (SYD) was identified as a co-activator of floral homeotic gene expression in Arabidopsis. SYD is also required for meristem maintenance and regulates flowering under a non-inductive photoperiod. SNF2 ATPases are structurally and functionally conserved from yeast to humans. In addition to the conserved protein features, SYD has a large unique C-terminal domain. We show here that SYD is present as two forms in the nucleus, full-length and truncated, with the latter apparently lacking the C-terminal domain. The ratio of the two forms of endogenous SYD differs in juvenile and in adult tissues. Furthermore, an SYD variant lacking the C-terminal domain (SYDΔC) rescues the syd null mutant, indicating that the N-terminal ATPase AT-hook-containing region of SYD is sufficient for biological activity. Plants expressing SYDΔC show molecular and morphological phenotypes opposite to those of the null mutant, suggesting that the construct results in increased activity. This increased activity is at least in part due to elevated SYD protein levels in these lines. We propose that the C-terminal domain may control SYD accumulation and/or specific activity in the context of the full-length protein. The presence of the C-terminal domain in rice SYD suggests that its role is probably conserved in the two classes of flowering plants.

Original languageEnglish (US)
Pages (from-to)685-699
Number of pages15
JournalPlant Journal
Volume46
Issue number4
DOIs
StatePublished - May 2006

Keywords

  • Arabidopsis
  • Chromatin remodeling
  • Development
  • SNF2/BRM
  • SPLAYED
  • Transcription

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