The nucleotide sequence of POX18, a gene encoding a small oleate-inducible peroxisomal protein from Candida tropicalis

Les J. Szabo; Gillian M. Small; Paul B. Lazarow

doi:10.1016/0378-1119(89)90388-0

The nucleotide sequence of POX18, a gene encoding a small oleate-inducible peroxisomal protein from Candida tropicalis

Les J. Szabo, Gillian M. Small, Paul B. Lazarow

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We report the molecular cloning and nucleotide sequence of the nuclear gene, POX18, encoding an oleate-inducible peroxisomal protein from the yeast Candida tropicalis. POX18 has a single open reading frame of 381 nucleotides (nt), which encodes a protein of 127 amino acids. The predicted M_r of this protein is 13 792. Codon usage in the expression of POX18 is non-random, and shows a pattern similar to that used for other peroxisomal genes from C. tropicalis and highly expressed genes from Saccharomyces cerevisiae. Northern analysis of total RNA from oleate-grown cells determined that POX18 mRNA is approximately 750 nt in length. The POX18 gene was expressed in vitro, which resulted in a single translation product that co-migrated in denaturing polyacrylamide gels with an abundant peroxisomal protein (apparent mass of 16 kDa) and was immunoprecipitated by an antiserum against peroxisomal protein.

Original language	English (US)
Pages (from-to)	119-126
Number of pages	8
Journal	Gene
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(89)90388-0
State	Published - Jan 30 1989
Externally published	Yes

Bibliographical note

Funding Information:
We gratefully acknowledge Mr. Gary Richo, Mr. David Allman and Mr. Bruce Fieldman for technical assistance, and Drs. Gary Otto and Anthony Po-powics for assistance with the computer analysis. This work was supported by National Science Foundation grant DCB83-09222 and National Institute of Health grant DK-39684.

Keywords

Recombinant DNA
codons
gene structure
hydropathy plot
in vitro expression

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title = "The nucleotide sequence of POX18, a gene encoding a small oleate-inducible peroxisomal protein from Candida tropicalis",

abstract = "We report the molecular cloning and nucleotide sequence of the nuclear gene, POX18, encoding an oleate-inducible peroxisomal protein from the yeast Candida tropicalis. POX18 has a single open reading frame of 381 nucleotides (nt), which encodes a protein of 127 amino acids. The predicted Mr of this protein is 13 792. Codon usage in the expression of POX18 is non-random, and shows a pattern similar to that used for other peroxisomal genes from C. tropicalis and highly expressed genes from Saccharomyces cerevisiae. Northern analysis of total RNA from oleate-grown cells determined that POX18 mRNA is approximately 750 nt in length. The POX18 gene was expressed in vitro, which resulted in a single translation product that co-migrated in denaturing polyacrylamide gels with an abundant peroxisomal protein (apparent mass of 16 kDa) and was immunoprecipitated by an antiserum against peroxisomal protein.",

keywords = "Recombinant DNA, codons, gene structure, hydropathy plot, in vitro expression",

author = "Szabo, {Les J.} and Small, {Gillian M.} and Lazarow, {Paul B.}",

note = "Funding Information: We gratefully acknowledge Mr. Gary Richo, Mr. David Allman and Mr. Bruce Fieldman for technical assistance, and Drs. Gary Otto and Anthony Po-powics for assistance with the computer analysis. This work was supported by National Science Foundation grant DCB83-09222 and National Institute of Health grant DK-39684.",

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AU - Lazarow, Paul B.

N1 - Funding Information: We gratefully acknowledge Mr. Gary Richo, Mr. David Allman and Mr. Bruce Fieldman for technical assistance, and Drs. Gary Otto and Anthony Po-powics for assistance with the computer analysis. This work was supported by National Science Foundation grant DCB83-09222 and National Institute of Health grant DK-39684.

PY - 1989/1/30

Y1 - 1989/1/30

N2 - We report the molecular cloning and nucleotide sequence of the nuclear gene, POX18, encoding an oleate-inducible peroxisomal protein from the yeast Candida tropicalis. POX18 has a single open reading frame of 381 nucleotides (nt), which encodes a protein of 127 amino acids. The predicted Mr of this protein is 13 792. Codon usage in the expression of POX18 is non-random, and shows a pattern similar to that used for other peroxisomal genes from C. tropicalis and highly expressed genes from Saccharomyces cerevisiae. Northern analysis of total RNA from oleate-grown cells determined that POX18 mRNA is approximately 750 nt in length. The POX18 gene was expressed in vitro, which resulted in a single translation product that co-migrated in denaturing polyacrylamide gels with an abundant peroxisomal protein (apparent mass of 16 kDa) and was immunoprecipitated by an antiserum against peroxisomal protein.

AB - We report the molecular cloning and nucleotide sequence of the nuclear gene, POX18, encoding an oleate-inducible peroxisomal protein from the yeast Candida tropicalis. POX18 has a single open reading frame of 381 nucleotides (nt), which encodes a protein of 127 amino acids. The predicted Mr of this protein is 13 792. Codon usage in the expression of POX18 is non-random, and shows a pattern similar to that used for other peroxisomal genes from C. tropicalis and highly expressed genes from Saccharomyces cerevisiae. Northern analysis of total RNA from oleate-grown cells determined that POX18 mRNA is approximately 750 nt in length. The POX18 gene was expressed in vitro, which resulted in a single translation product that co-migrated in denaturing polyacrylamide gels with an abundant peroxisomal protein (apparent mass of 16 kDa) and was immunoprecipitated by an antiserum against peroxisomal protein.

KW - Recombinant DNA

KW - codons

KW - gene structure

KW - hydropathy plot

KW - in vitro expression

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The nucleotide sequence of POX18, a gene encoding a small oleate-inducible peroxisomal protein from Candida tropicalis

Abstract

Bibliographical note

Keywords

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