The purinergic receptor P2RX7 directs metabolic fitness of long-lived memory CD8 + T cells

Henrique Borges Da Silva, Lalit K. Beura, Haiguang Wang, Eric A. Hanse, Reshma Gore, Milcah C. Scott, Daniel A. Walsh, Katharine E. Block, Raissa Fonseca, Yan Yan, Keli L. Hippen, Bruce R. Blazar, David Masopust, Ameeta Kelekar, Lucy Vulchanova, Kristin A. Hogquist, Stephen C. Jameson

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


Extracellular ATP (eATP) is an ancient 'danger signal' used by eukaryotes to detect cellular damage 1 . In mice and humans, the release of eATP during inflammation or injury stimulates both innate immune activation and chronic pain through the purinergic receptor P2RX7 2-4 . It is unclear, however, whether this pathway influences the generation of immunological memory, a hallmark of the adaptive immune system that constitutes the basis of vaccines and protective immunity against re-infection 5,6 . Here we show that P2RX7 is required for the establishment, maintenance and functionality of long-lived central and tissue-resident memory CD8+ T cell populations in mice. By contrast, P2RX7 is not required for the generation of short-lived effector CD8+ T cells. Mechanistically, P2RX7 promotes mitochondrial homeostasis and metabolic function in differentiating memory CD8+ T cells, at least in part by inducing AMP-activated protein kinase. Pharmacological inhibitors of P2RX7 provoked dysregulated metabolism and differentiation of activated mouse and human CD8+ T cells in vitro, and transient P2RX7 blockade in vivo ameliorated neuropathic pain but also compromised production of CD8+ memory T cells. These findings show that activation of P2RX7 by eATP provides a common currency that both alerts the nervous and immune system to tissue damage, and promotes the metabolic fitness and survival of the most durable and functionally relevant memory CD8+ T cell populations.

Original languageEnglish (US)
Pages (from-to)264-268
Number of pages5
Issue number7713
StatePublished - Jul 12 2018

Bibliographical note

Funding Information:
Acknowledgements We thank the UMN Flow Cytometry Resource for cell sorting, C. Henzler (UMN Supercomputing Institute) for bioinformatics analysis, F. Zhou (UMN Characterization Facility) for transmission electron microscopy, M. Pierson for viral plaque assays, the NIH Tetramer Core for peptide/MHC tetramers, and A. Goldrath, S. Kaech, G. Shadel, R. Jones, E. Pearce, M. Jenkins, V. Vezys and members of the Jamequist laboratory and UMN Center for Immunology for discussions. The UMN Characterization Facility is a member of the NSF-funded Materials Research Facilities Network ( via the MRSEC program. This work was supported by NIH grants AI38903 and AI75168 (S.C.J.), CA157971 (A.K.), and MN Partnership Infrastructure Award MNPIF#16.09 (A.K.). H.B.d.S. was supported by a CNPq research fellowship from the Ministry of Science, Technology and Innovation of Brazil.

Publisher Copyright:
© 2018 Macmillan Publishers Ltd., part of Springer Nature.


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