Abstract
Ribosomes in lysates prepared from the mycelia of Aspergillus giganteus MDH 18894, which are actively secreting α-sarcin, do not contain the α-sarcin lesion. However, the addition of exogenous α-sarcin to these same lysates results in cleavage of the 26 S rRNA of the 60 S ribosomal subunit, characteristic of the cytotoxic action of α-sarcin. We conclude that A. giganteus ribosomes are not inherently resistant to the action of α-sarcin but are protected in vivo by producing α-sarcin in an inactive form and/or by the efficient cotranslational secretion of the toxin.
Original language | English (US) |
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Pages (from-to) | 388-390 |
Number of pages | 3 |
Journal | FEBS Letters |
Volume | 229 |
Issue number | 2 |
DOIs | |
State | Published - Mar 14 1988 |
Bibliographical note
Copyright:Copyright 2014 Elsevier B.V., All rights reserved.
Keywords
- (Aspergillus giganteus)
- Ribosome inactivation
- Ribosome-inactivating protein
- α-Sarcin