The ribosomes of Aspergillus giganteus are sensitive to the cytotoxic action of α-sarcin

Stephen P. Miller, James W. Bodley

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Ribosomes in lysates prepared from the mycelia of Aspergillus giganteus MDH 18894, which are actively secreting α-sarcin, do not contain the α-sarcin lesion. However, the addition of exogenous α-sarcin to these same lysates results in cleavage of the 26 S rRNA of the 60 S ribosomal subunit, characteristic of the cytotoxic action of α-sarcin. We conclude that A. giganteus ribosomes are not inherently resistant to the action of α-sarcin but are protected in vivo by producing α-sarcin in an inactive form and/or by the efficient cotranslational secretion of the toxin.

Original languageEnglish (US)
Pages (from-to)388-390
Number of pages3
JournalFEBS Letters
Volume229
Issue number2
DOIs
StatePublished - Mar 14 1988

Keywords

  • (Aspergillus giganteus)
  • Ribosome inactivation
  • Ribosome-inactivating protein
  • α-Sarcin

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