The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants

Anshu Alok, Dulam Sandhya, Phanikanth Jogam, Vandasue Rodrigues, Kaushal K. Bhati, Himanshu Sharma, Jitendra Kumar

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations

Abstract

Cpf1, an endonuclease of the class 2 CRISPR family, fills the gaps that were previously faced in the world of genome engineering tools, which include the TALEN, ZFN, and CRISPR/Cas9. Other simultaneously discovered nucleases were not able to carry out re-engineering at the same region due to the loss of a target site after first-time engineering. Cpf1 acts as a dual nuclease, functioning as an endoribonuclease to process crRNA and endodeoxyribonuclease to cleave target sequences and generate double-stranded breaks. Additionally, Cpf1 allows for multiplexed genome editing, as a single crRNA array transcript can target multiple loci in the genome. The CRISPR/Cpf1 system enables gene deletion, insertion, base editing, and locus tagging in monocot as well as in dicot plants with fewer off-target effects. This tool has been efficiently demonstrated into tobacco, rice, soybean, wheat, etc. This review covers the development and applications of Cpf1 mediated genome editing technology in plants.

Original languageEnglish (US)
Article number264
JournalFrontiers in Plant Science
Volume11
DOIs
StatePublished - Mar 31 2020

Keywords

  • CRISPR
  • Cas9
  • Cpf1
  • NHEJ
  • TALEN
  • ZFN
  • crRNA
  • endoribonuclease

PubMed: MeSH publication types

  • Journal Article
  • Review

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