Abstract
Four bacterial isolates were examined for their ability to increase the availability of water soluble Cu, Cr, Pb and Zn in soils and for their effect on metals uptake by Zea mays and Sorghum bicolor. Random Amplified Polymorphic DNA (RAPD) analysis was used to show that the bacterial cultures were genetically diverse. Bacterial isolates S3, S28, S22 and S29 had 16S rRNA gene sequences that were most similar to Bacillus subtilis, Bacillus pumilus, Pseudomonas pseudoalcaligenes and Brevibacterium halotolerans based on 100% similarity in their 16S rDNA gene sequence, respectively. Filtrate liquid media that had supported B. pumilus and B. subtilis growth significantly increased Cr and Cu extraction from soil polluted with tannery effluent and from Cu-rich soil, respectively, compared to axenic media. The highest concentrations of Pb (0.2 g kg-1), Zn (4 g kg-1) and Cu (2 g kg-1) were accumulated in shoots of Z. mays grown on Cu-rich soil inoculated with Br. halotolerans. The highest concentration of Cr (5 g kg-1) was accumulated in S. bicolor roots grown in tannery-effluent-polluted soil inoculated with a mixed inoculum of bacterial strains. These results show that bacteria play an important role in increasing metal availability in soil, thus enhancing Cr, Pb, Zn and Cu accumulation by Z. mays and S. bicolor.
Original language | English (US) |
---|---|
Pages (from-to) | 253-262 |
Number of pages | 10 |
Journal | World Journal of Microbiology and Biotechnology |
Volume | 24 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2008 |
Bibliographical note
Funding Information:Partially funded by SAF2014-54371 ( FEDER funds), SEPAR 2015 and 2016, FUCAP 2014, and Menarini 2015.
Funding Information:
Partially funded by SAF2014-54371 (FEDER funds), SEPAR 2015 and 2016, FUCAP 2014, and Menarini 2015.
Keywords
- 16S rRNA
- Bacteria
- Metal uptake
- RAPD
- Sorghum bicolor
- Zea mays