The role of nitrate reduction in the anoxic metabolism of roots II. Anoxic metabolism of tobacco roots with or without nitrate reductase activity

M. Stoimenova, I. G.L. Libourel, R. G. Ratcliffe, W. M. Kaiser

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The effects of root anoxia on a tobacco (Nicotiana tabacum) wild type (WT) and a transformant (LNR-H) lacking root nitrate reductase were compared. LNR-H plants were visibly more sensitive to oxygen deprivation than WT, showing rapid and heavy wilting symptoms. LNR-H roots also produced substantially more ethanol and lactate than WT roots under anoxia, and their sugar and sugar-P content, as well as their ATP levels, remained higher. The fermentation rates of WT and LNR-H roots were unaffected by sugar feeding and the higher fermentation rate in the LNR-H roots was associated with a greater acidification of the cytoplasm under anoxia. From these observations it is concluded: (i) that the absence of NR activity in the LNR-H roots does not necessarily limit NADH recycling; and (ii) that nitrate reduction in the WT roots results in a more acidifying metabolism. It is the higher metabolic rate in the LNR-H roots that leads to the greater cytoplasmic acidification under anoxia despite the absence of a contribution from the metabolism of nitrate. Competition for NADH cannot explain this difference in metabolic rate, and it remains unclear why the NR-free LNR-H, and tungstate-treated WT roots, had much higher fermentation rates than WT roots. The difference in anaerobic metabolism could still be due to the presence or absence of nitrate reductase and the possibility that this could occur through the production of nitric oxide is discussed.

Original languageEnglish (US)
Pages (from-to)155-167
Number of pages13
JournalPlant and Soil
Volume253
Issue number1
DOIs
StatePublished - Jun 2003

Bibliographical note

Funding Information:
This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG Ka 456/13-1) to WMK. The skilled technical assistance of Maria Lesch and Eva Wirth is gratefully acknowledged. IGLL and RGR thank the UK Biotechnology and Biological Sciences Research Council and Aventis CropScience for financial support.

Keywords

  • Anoxia
  • Cytoplasmic pH
  • Fermentation
  • NMR spectroscopy
  • Nitrate reductase
  • Root metabolism

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