Abstract
We have previously described significant changes in skin differentiation and the delay in wound healing from delta-opioid receptor knockout mice. In addition, we have shown that opioid receptor ligands and their receptor systems affect wound healing in vitro and the migration pattern of human skin cells, such as keratinocytes and fibroblasts (Bigliardi-Qi et al., Differentiation 74:174–185, 2006; Bigliardi et al., Exp Dermatol 18:424–430, 2009; Bigliardi et al., J Recept Signal Transduct Res 22:191–199, 2002). This observation is true for both primary keratinocytes and fibroblasts derived from foreskin or normal human skin as well as for immortalized cell lines such as HaCaT cells.
Original language | English (US) |
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Pages (from-to) | 273-277 |
Number of pages | 5 |
Journal | Methods in Molecular Biology |
Volume | 1230 |
DOIs | |
State | Published - 2015 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2015.
Keywords
- Delta-opioid receptor
- Fibroblast
- Keratinocyte
- Wound scratch assay