The sccmec types and antimicrobial resistance among methicillin-resistant staphylococcus species isolated from dogs with superficial pyoderma

Yuttana Chanayat; Areerath Akatvipat; Jeff B. Bender; Veerasak Punyapornwithaya; Tongkorn Meeyam; Usanee Anukool; Duangporn Pichpol

doi:10.3390/vetsci8050085

The sccmec types and antimicrobial resistance among methicillin-resistant staphylococcus species isolated from dogs with superficial pyoderma

Yuttana Chanayat, Areerath Akatvipat, Jeff B. Bender, Veerasak Punyapornwithaya, Tongkorn Meeyam, Usanee Anukool, Duangporn Pichpol

Environmental Health Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.

Original language	English (US)
Article number	85
Journal	Veterinary Sciences
Volume	8
Issue number	5
DOIs	https://doi.org/10.3390/vetsci8050085
State	Published - May 2021

Bibliographical note

Funding Information:
Funding: This research was funded by the Project of Antibiotic Stewardship in Companion Animal Practice jointly with Chiang Mai University/the University of Minnesota, College of Veterinary Medicine (R000012898), Excellent Center of Veterinary Public Health (R000016652), and Research Group for Veterinary Public Health (R000026062), Faculty of Veterinary Medicine, Chiang Mai University, Thailand.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

MRS
Pyoderma
Skin swab
Staphylococcal infection
Type V SCCmec
Zoonotic disease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "The sccmec types and antimicrobial resistance among methicillin-resistant staphylococcus species isolated from dogs with superficial pyoderma",

abstract = "This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.",

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note = "Funding Information: Funding: This research was funded by the Project of Antibiotic Stewardship in Companion Animal Practice jointly with Chiang Mai University/the University of Minnesota, College of Veterinary Medicine (R000012898), Excellent Center of Veterinary Public Health (R000016652), and Research Group for Veterinary Public Health (R000026062), Faculty of Veterinary Medicine, Chiang Mai University, Thailand. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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N2 - This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.

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The sccmec types and antimicrobial resistance among methicillin-resistant staphylococcus species isolated from dogs with superficial pyoderma

Abstract

Bibliographical note

Keywords

UN SDGs

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