TY - JOUR
T1 - The Schizosaccharomyces pombe gene encoding γ-glutamyl transpeptidase I is regulated by non-fermentable carbon sources and nitrogen starvation
AU - Kim, Hong Gyum
AU - Park, Hey Jung
AU - Kang, Hyun Jung
AU - Lim, Hye Won
AU - Kim, Kyunghoon
AU - Park, Eun Hee
AU - Ahn, Kisup
AU - Lim, Chang Jin
PY - 2005/2/1
Y1 - 2005/2/1
N2 - In our previous study, the first structural gene (GGTI) encoding γ-glutamyl transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of β-galactosidase from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.
AB - In our previous study, the first structural gene (GGTI) encoding γ-glutamyl transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of β-galactosidase from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.
KW - Acetate
KW - Ethanol
KW - GGTI-lacZ fusion gene
KW - Nitrogen starvation
KW - Non-fermentable carbon sources
KW - Pap1
KW - Regulation
KW - Schizosaccharomyces pombe
KW - γ-glutamyl transpeptidase
UR - http://www.scopus.com/inward/record.url?scp=15944406353&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15944406353&partnerID=8YFLogxK
M3 - Article
C2 - 15765057
AN - SCOPUS:15944406353
SN - 1225-8873
VL - 43
SP - 44
EP - 48
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 1
ER -