The Schizosaccharomyces pombe gene encoding γ-glutamyl transpeptidase I is regulated by non-fermentable carbon sources and nitrogen starvation

Hong Gyum Kim, Hey Jung Park, Hyun Jung Kang, Hye Won Lim, Kyunghoon Kim, Eun Hee Park, Kisup Ahn, Chang Jin Lim

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In our previous study, the first structural gene (GGTI) encoding γ-glutamyl transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of β-galactosidase from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalJournal of Microbiology
Volume43
Issue number1
StatePublished - Feb 1 2005

Keywords

  • Acetate
  • Ethanol
  • GGTI-lacZ fusion gene
  • Nitrogen starvation
  • Non-fermentable carbon sources
  • Pap1
  • Regulation
  • Schizosaccharomyces pombe
  • γ-glutamyl transpeptidase

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