The sub cellular localization of stereo specific opiate binding in mouse brain

A. P. Smith, H. H. Loh

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Of the three primary sub cellular fractions derived from mouse brain homogenates, the P3 (microsomal) fraction binds 3H di hydromorphine stereo specifically with the highest capacity per mg. of protein; this level is nearly as great as that bound by purified synaptosomal plasma membranes (SPM). The binding to P3 is unlikely to be attributable to contamination with SPM, because a) ten times as much total binding is recovered in P3 as in SPM, and b) the level of binding to P3 is highest in a sub fraction banding above 0.8 M sucrose, rich in surface membranes of all types, whereas SPM bands preferentially at 0.8-1.1 M sucrose. The binding of either 3H di hydromorphine or 3H naloxone to P3 is, however, indistinguishable from that found in nerve endings with respect to a) its K(D); b) the relative potencies of several agonists in displacing it; and c) the effects on it of Na+ or trypsin. Thus, it appears that stereo specific opiate receptors are distributed diffusely on the entire surface of nerve cells and not concentrated at the synaptic region as has previously been supposed.

Original languageEnglish (US)
Pages (from-to)205-219
Number of pages15
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume15
Issue number2
StatePublished - Dec 1 1976

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