Allelic polymorphism in TCR loci may play an important role in shaping the T cell repertoire and in disease susceptibility. We have used a combination of antibody and sequence analysis to investigate polymorphism in the murine Vα11 family. Two different antibodies have been analyzed that recognize particular Vα11 family members of the Vαb and Vα(d) haplotypes. One antibody shows Jα dependency, suggesting a conformational element to the epitope. Investigation of the anti-Vα11 staining pattern on different mouse strains indicates that there is a marked influence of MHC haplotype on Vα11 selection and that Vα11 is preferentially expressed on CD4+ cells. Sequence analysis of Vα11 genes from the Vαa, Vαb, and Vα(d) haplotypes shows two potential regions for the haplotype-specific epitopes. The relatedness of the different Vα11 family members from different haplotypes suggests that the Vα11.1/11.2 gene duplication is relatively recent, but that Vα11.3 separated much earlier. Differences between Vα11.3 and Vα11.1/11.2 are concentrated in the putative complementarity determining regions (CDR), whereas differences between alleles are not clearly clustered. However, the Vα11.1a and Vα11.1(d) alleles differ from Vα11.1b and Vα11.2b in CDR1. A Vα11.2-expressing anti-cytochrome c T cell has the same V-J junction as a Vα11.1-bearing cell with a similar fine specificity, indicating that Vα11.1b and Vα11.2b do not contribute different Ag specificities.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Immunology|
|State||Published - 1991|