TY - JOUR
T1 - The Xenopus oocyte
T2 - a single-cell model for studying Ca2+ signaling.
AU - Lin-Moshier, Yaping
AU - Marchant, Jonathan S.
PY - 2013/3
Y1 - 2013/3
N2 - In the four decades since the Xenopus oocyte was first demonstrated to have the capacity to translate exogenous mRNAs, this system has been exploited for many different experimental purposes. Typically, the oocyte is used either as a "biological test tube" for heterologous expression of proteins without any particular cell biological insight or, alternatively, it is used for applications where cell biology is paramount, such as investigations of the cellular adaptations that power early development. In this article, we discuss the utility of the Xenopus oocyte for studying Ca2+ signaling in both these contexts.
AB - In the four decades since the Xenopus oocyte was first demonstrated to have the capacity to translate exogenous mRNAs, this system has been exploited for many different experimental purposes. Typically, the oocyte is used either as a "biological test tube" for heterologous expression of proteins without any particular cell biological insight or, alternatively, it is used for applications where cell biology is paramount, such as investigations of the cellular adaptations that power early development. In this article, we discuss the utility of the Xenopus oocyte for studying Ca2+ signaling in both these contexts.
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U2 - 10.1101/pdb.top066308
DO - 10.1101/pdb.top066308
M3 - Article
C2 - 23457336
AN - SCOPUS:84874704380
SN - 1559-6095
VL - 8
SP - 185
EP - 191
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 3
ER -