Thiostrepton-resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit

Sidney Pestka; David Weiss; Robert Vince; Bertold Wienen; Georg Stöffler; Issar Smith

doi:10.1007/BF00341721

Thiostrepton-resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit

Sidney Pestka, David Weiss, Robert Vince, Bertold Wienen, Georg Stöffler, Issar Smith

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Abstract

A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes and S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [³H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and-resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.

Original language	English (US)
Pages (from-to)	235-241
Number of pages	7
Journal	MGG Molecular & General Genetics
Volume	144
Issue number	3
DOIs	https://doi.org/10.1007/BF00341721
State	Published - Jan 1976
Externally published	Yes

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title = "Thiostrepton-resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit",

abstract = "A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes and S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and-resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.",

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T2 - Localization of resistance to the 50S subunit

AU - Pestka, Sidney

AU - Weiss, David

AU - Vince, Robert

AU - Wienen, Bertold

AU - Stöffler, Georg

AU - Smith, Issar

PY - 1976/1

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AB - A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes and S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and-resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.

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Thiostrepton-resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit

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