Abstract
Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an l-arginine affinity column (Kd ≈ 0.2–0.4 mM). The binding sites contain specific internal and bulge loops, whose sequences can include arginine coding triplets. Binding is highly specific for arginine, but all three motifs, like the self-splicing group I intron, also bind guanosine 5′-monophosphate. One site is stereoselective, somewhat preferring d-arginine.
Original language | English (US) |
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Pages (from-to) | 5497-5502 |
Number of pages | 6 |
Journal | Biochemistry |
Volume | 32 |
Issue number | 21 |
DOIs | |
State | Published - 1993 |