Thymidine-analog and multi-nucleoside resistance mutations are observed in both zidovudine-naive and zidovudine-experienced subjects with viremia after treatment with stavudine-containing regimens

L. Ross, William K Henry, D. Paar, P. Salvato, M. Shaefer, R. Fisher, Q. Liao, M. St. Clair

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Objective: The type and frequency of mutations in the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase coding region observed in virus from antiretroviral therapy (ART)-experienced, zidovudine (ZDV)-naive subjects receiving stavudine (d4T)-based therapies were compared with mutations observed in virus from ART-experienced subjects with previous ZDV exposure. Methods: Plasma HIV-1 RNA was isolated from 67 ART-experienced subjects. Reverse transcriptase mutations were assessed by sequencing polymerase chain reaction products. Results: Thirty-four subjects (51%) were ZDV-experienced (Zexp) and 33 (49%) were ZDV-naive and d4T-experienced (dexpZnaive). Human immunodeficiency virus type 1 from 16 of 33 (48%) dexpZnaive subjects and from 16 of 34 (47%) Zexp subjects had thymidine analog mutations (TAMs). Multinucleoside resistance (MNR) mutations were observed in virus from 5 of 33 (15%) dexpZnaive subjects and 3 of 34 (9%) Zexp subjects. At least one TAM or MNR mutation was identified in 18 of 33 (55%) of the former and in 19 of 34 (56%) of the latter group. Conclusions: These results confirm recent reports that TAMs and MNR mutations can arise in subjects receiving d4T-based therapy who are naive with respect to ZDV.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalJournal of Human Virology
Volume4
Issue number4
StatePublished - Nov 24 2001

Keywords

  • D4T
  • Mutations
  • Stavudine
  • ZDV
  • Zidovudine

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