TY - JOUR
T1 - Thymidine analogs are transferred from prelabeled donor to host cells in the central nervous system after transplantation
T2 - A word of caution
AU - Burns, Terry C.
AU - Ortiz-González, Xilma R.
AU - Gutiérrez-Pérez, María
AU - Keene, C. Dirk
AU - Sharda, Rohit
AU - Demorest, Zachary L.
AU - Jiang, Yuehua
AU - Nelson-Holte, Molly
AU - Soriano, Mario
AU - Nakagawa, Yasushi
AU - Luquin, María Rosario
AU - Garcia-Verdugo, Jose Manuel
AU - Prósper, Felipe
AU - Low, Walter C.
AU - Verfaillie, Catherine M.
PY - 2006/4
Y1 - 2006/4
N2 - Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label dividing cells by incorporating into DNA during S phase of cell division and are widely employed to identify cells transplanted into the central nervous system. However, the potential for transfer of thymidine analogs from grafted cells to dividing host cells has not been thoroughly tested. We here demonstrate that graft-derived thymidine analogs can become incorporated into host neural precursors and glia. Large numbers of labeled neurons and glia were found 3-12 weeks after transplantation of thymidine analog-labeled live stem cells, suggesting differentiation of grafted cells. Remarkably, however, similar results were obtained after transplantation of dead cells or labeled fibroblasts. Our findings reveal for the first time that thymidine analog labeling may not be a reliable means of identifying transplanted cells, particularly in highly proliferative environments such as the developing, neurogenic, or injured brain.
AB - Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label dividing cells by incorporating into DNA during S phase of cell division and are widely employed to identify cells transplanted into the central nervous system. However, the potential for transfer of thymidine analogs from grafted cells to dividing host cells has not been thoroughly tested. We here demonstrate that graft-derived thymidine analogs can become incorporated into host neural precursors and glia. Large numbers of labeled neurons and glia were found 3-12 weeks after transplantation of thymidine analog-labeled live stem cells, suggesting differentiation of grafted cells. Remarkably, however, similar results were obtained after transplantation of dead cells or labeled fibroblasts. Our findings reveal for the first time that thymidine analog labeling may not be a reliable means of identifying transplanted cells, particularly in highly proliferative environments such as the developing, neurogenic, or injured brain.
KW - Adult bone marrow stem cells
KW - Bromodeoxyuridine
KW - Control
KW - In vivo tracking
KW - Label
KW - Neural differentiation
KW - Thymidine analog
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=33744998969&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744998969&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2005-0463
DO - 10.1634/stemcells.2005-0463
M3 - Article
C2 - 16373692
AN - SCOPUS:33744998969
SN - 1066-5099
VL - 24
SP - 1121
EP - 1127
JO - STEM CELLS
JF - STEM CELLS
IS - 4
ER -