Tissue-engineered valves with commissural alignment

M. R. Neidert, P. S. Robinson, S. L. Johnson, R. T. Tranquillo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel approach to producing bioartificial valves using the tissue-equivalent method of entrapping cells within a biopolymer gel and using a mold design that presents appropriate mechanical constraints to the cell induced gel compaction so as to yield both the fibril alignment and the geometry of a native valve. Producing a bioartificial valve that has the same fibril alignment as the native valve is believed to be important in realizing mechanical properties similar to the native valve. Bileaflet valves were fabricated from bovine collagen and neonatal human dermal fibroblasts as proof of principle. The resultant valves possessed both commissure-to-commissure alignment of collagen fibers in the leaflets and circumferential alignment in the root. The leaflet alignment was reflected in anisotropic tensile properties. Histology of the leaflets, however, revealed an aligned collagen matrix with very little of the other ECM components present in the native valve. Consequently, valves were fabricated from fibrin instead of collagen, since fibrin has been shown to stimulate matrix production by entrapped tissue cells.

Original languageEnglish (US)
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
StatePublished - Dec 1 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

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