TNF-α stimulates the biosynthesis of complement C3 and factor B by human umbilical cord vein endothelial cells

Yasuhiko Kawakami, Yoshiki Watanabe, Masahide Yamaguchi, Haruko Sakaguchi, Ichiro Kono, Ayako Ueki

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19 Scopus citations

Abstract

The human umbilical cord vein (HUVEC) endothelial cells synthesize and secrete complement components. We analyzed the regulation of biosynthesis of the third component of complement (C3) and factor B (Bf) by cytokines in endothelial cells. Production of C3 increased after stimulation with TNF-α or IL-1β and that of Bf with TNF-α, TNF-β and IFN-γ. TNF-α was the most effective in stimulating the secretion of C3 and Bf or the expression of mRNA of C3 or Bf. Preincubation with TNF-α for at least 36 or 24 h was needed for the stimulation, respectively. TNF-α, together with IFN-γ had synergistic effects and the release of C3 or Bf increased to about 40- and 26-fold higher than those in control cells after incubation with both cytokines.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalCancer Letters
Volume116
Issue number1
DOIs
StatePublished - Jun 3 1997

Bibliographical note

Funding Information:
We wish to express our appreciation to Dr. R.D. Campbell, MRC Immunochemistry Unit, Oxford, for providing us a human Bf cDNA probe. The authors also thank Dr. T. Nohno in Kawasaki Med. School and Dr. O. Tokunaga in Saga Med. School for their kind advice. This work was partly supported by a Grant-in Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and by Kawasaki Medical School Research Project Grant.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

  • Complement
  • Cytokines
  • Endothelial cells

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